IFN-α/β/IFN-γ/IL-15 pathways identify GBP1-expressing tumors with an immune-responsive phenotype

Immunotherapy is widely used in cancer treatment; however, only a subset of patients responds well to it. Significant efforts have been made to identify patients who will benefit from immunotherapy. Successful anti-tumor immunity depends on an intact cancer-immunity cycle, especially long-lasting CD8+ T-cell responses. Interferon (IFN)-α/β/IFN-γ/interleukin (IL)-15 pathways have been reported to be involved in the development of CD8+ T cells. And these pathways may predict responses to immunotherapy. Herein, we aimed to analyze multiple public databases to investigate whether IFN-α/β/IFN-γ/IL-15 pathways could be used to predict the response to immunotherapy. Results showed that IFN-α/β/IFN-γ/IL-15 pathways could efficiently predict immunotherapy response, and guanylate-binding protein 1 (GBP1) could represent the IFN-α/β/IFN-γ/IL-15 pathways. In public and private cohorts, we further demonstrated that GBP1 could efficiently predict the response to immunotherapy. Functionally, GBP1 was mainly expressed in macrophages and strongly correlated with chemokines involved in T-cell migration. Therefore, our study comprehensively investigated the potential role of GBP1 in immunotherapy, which could serve as a novel biomarker for immunotherapy and a target for drug development. Supplementary Information The online version contains supplementary material available at 10.1007/s10238-024-01328-w.


Introduction
Recently, immunotherapy targeting the PD-1/PD-L1 pathway has significantly improved T-cell-mediated anti-tumor responses and induced objective clinical responses in multiple tumor types.However, only a minority of patients experience durable survival after treatment with therapies.To maximize treatment benefits, predictive biomarkers, such as PD-L1 and tumor mutational burden (TMB), have been developed to guide therapeutic decisions [1].However, data from clinical trials have demonstrated that patients with low PD-L1 expression could still respond to immunotherapy [2,3].In addition, currently used single biomarkers ignore the complex immune responses that occur in the tumor microenvironment (TME).Hence, precise prediction of immunotherapy requires further investigation.
The TME plays a crucial role in cancer progression and therapeutic responses [4].Tumor-infiltrating immune cells, especially CD8 + T cells, can profoundly influence tumor progression and success of anticancer therapies [5].Previously, T-cell infiltration has been linked to the response to immunotherapy, specifically the immune checkpoint blockade (ICB) [6,7].Successful and long-term immunotherapeutic responses depend on CD8 + T-cell activation, cancer cell killing, and memory CD8 + T-cell maintenance [8].These steps require distinct signaling pathways: (1) Interferon (IFN)-α/β pathway: Type I IFN could boost cDC1 cross-priming and CD8 + T-cell reactivation [9]; (2) IFN-γ pathway: Cytotoxic CD8 + T cells typically secret large amounts of IFN-γ to kill tumorigenic cells [10]; and (3) interleukin (IL)-15 pathway: IL-15 signaling provides a sustained immune response and maintains the memory phenotype of CD8 + T cells [11,12].Disruption of these steps may hinder the enduring effects of immunotherapy.Therefore, a comprehensive understanding of the TME could help improve immunotherapeutic strategies.
To address this need, we analyzed the IFN-α/β/IFN-γ/ IL-15 pathways to identify a potential combinatorial biomarker to predict responses to ICB.Bulk and single-cell RNA-seq data were collected and analyzed from multiple studies and cancer types, and results showed that a single IFN-α/β/IFN-γ/IL-15 pathway could predict clinical responses and survival to ICB.We found that GBP1 could represent and positively correlate with these three pathways, where its expression also positively correlated with the hot immune microenvironment.Further analysis showed that GBP1 mRNA and protein expression in baseline tumor tissues correlated with improved response and overall survival (OS) in multiple cohorts.Cumulatively, this study revealed that GBP1 could serve as a predictive biomarker and may improve the predictive outcomes of patients receiving ICB.

Data collection of bulk and single-cell RNA-seq cohorts
Bulk RNA-seq data from four public immune checkpoint inhibitor (ICI)-treated melanoma cohorts [13][14][15][16] that contained 286 pretreated patients were collected to validate the association between selected signaling pathways, GBP1, and the efficacy of immunotherapy.All patients with melanoma were treated with anti-PD-1 or anti-CTLA-4 antibodies.To calculate the correlation between selected signaling pathways and GBP1 expression in non-small cell lung cancer (NSCLC), four public cohorts with 417 patients with NSCLC were retrieved from the Gene Expression Omnibus (GEO) database (accession numbers: GSE81089, GSE103584, GSE112996, and GSE181820).In addition, transcriptomic data from 10,148 cases comprising 33 tumor types were downloaded from The Cancer Genome Atlas (TCGA) to investigate the correlation between selected signaling pathways and GBP1 expression, as well as the TME.
To evaluate the abundance of GBP1 in various cell populations, two cohorts of NSCLC [17] and melanoma [18], and a pan-cancer cohort of 13 tumor types [19], were used in this study.

Definition of the enrichment of selected signaling pathways
Three anti-tumor immune signaling pathways, including INF-α/β (REACTOME_INTERFERON_ALPHA_BETA_ SIGNALING), INF-γ (BIOCARTA_IFNG_PATHWAY), and IL-15 (REACTOME_INTERLEUKIN_15_SIGNAL-ING), were derived from the MSigDB database [20,21] and quantified via single-sample Gene Set Enrichment Analysis (ssGSEA) [22].In each cohort, patients were classified into high and low groups using the median enrichment score of each pathway as the threshold.The pooled cohort comprised the group information from each cohort.

Identification of GBP1 as an indicator for selected signaling pathways
To explore whether the activity within three selected signaling pathways could be represented by a single gene.The ssGSEA scores of above three selected signaling pathways were calculated for each tumor sample.Subsequently, Pearson's correlation coefficient was employed to assess the relationship between the expression level of each gene and the ssGSEA score corresponding to each chosen pathway across various tumor types.For each gene, the number of tumor types demonstrating a significantly positive correlation coefficient (r > 0 and p value ≤ 0.05) was aggregated.The genes were ranked based on the quantity of tumor types they were significantly associated with.

Survival and treatment response analysis
For the ICI-treated cohorts, patients were classified into high and low groups using the median enrichment scores of three selected signaling pathways, the median expression value of GBP1, or the median ssGSEA scores of six hot-/cold-related gene sets [23][24][25][26][27][28].The pooled cohort comprised the group information from each cohort.Survival curves were generated using the Kaplan-Meier method with survival package v.2.44 and visualized using the survminer package.Statistical significance between two groups was assessed using the log-rank test.Patients who survived longer than 36 months were defined as having long-term survival, and the percentage of patients with long-term survival analyzed.
The ICI treatment response was defined based on RECIST1.1.The objective response rate (ORR) was defined as the percentage of patients who achieved a complete or partial response.Responders were defined as patients who achieved an objective response or maintained stable disease for more than 6 months.The ORR and percentage of responders were analyzed in the individual ICI-treated and pooled cohorts.

Correlation analysis
For the TCGA data, GEO cohorts, and ICI-treated cohorts, the ssGSEA scores of the three selected signaling pathways were calculated for each tumor sample.The expression levels of GBP1, CXCL9, CXCL10, and CCL5 were normalized using log transformation.Correlation coefficients were calculated using Pearson's correlation coefficient.

Tumor microenvironment analysis
For TCGA data, patients were initially classified according to the median expression level of GBP1 in each tumor type.Thereafter, classification information for each tumor type was summarized to assign patients to high and low groups.The high group represented patients with a higher transcriptomic level of GBP1 than that of the median for each tumor type, and the low group represented patients with a lower transcriptomic level of GBP1 than that of the median.
For the "pro-to-anti-inflammatory cytokine ratio," the ratio of the average expression level (log-transformed FPKM value) of pro-inflammatory cytokines (IFN-γ, IL-1, and IL-2) versus anti-inflammatory cytokines (TGFB1, IL-10, IL-4, and IL-11) was calculated in each tumor sample.To calculate the ratio of CD8 + T cells versus Tregs, M1 macrophages, and myeloid-derived suppressor cells (MDSCs), the ratio of the average expression levels (logtransformed FPKM value) of the gene set for the corresponding cell types was calculated.
To determine the "hot" or "cold" microenvironment for the high and low groups, six hot-/cold-related gene sets were collected from the public databases used in this study.Scores were calculated using ssGSEA.
The Wilcoxon signed-rank test was used to compare the distribution of values between tumors with high and low GBP1 expression levels.The calculated ratios or scores of each tumor sample were standardized among samples from the same tumor type.

Single-cell RNA-seq analysis
The human melanoma and NSCLC scRNA-seq datasets were downloaded from the GEO database (accession numbers: GSE115978 and GSE117570).Quality control measures were applied to the cells.After filtering, the UMI count matrix was normalized by using the "Normalize-Data" function with default parameters.For the clustering of all cell types, 50 principal component analyses were applied to reduce dimensionality using the "FindClusters" function on 50 principal components (PCs) with a 1.2 resolution in GSE115978 and 0.4 in GSE117570, and each cluster annotated with known markers.Uniform Manifold Approximation and Projection (UMAP) using the "RunU-MAP" function was performed for visualization in two dimensions with the same PCs.GBP1 expression was visualized via feature and violin plots using the "FeaturePlot" and "VlnPlot" functions in all cell types.
For the pan-cancer cohort, raw count data were normalized using the "NormalizedData" function from Seurat [29] with default parameters.Cell-type information for each cell was obtained from the public databases.Violin plots of the normalized expression levels of GBP1 in each cell type were obtained using the "VlnPlot" function.

Enrichment analysis
For TCGA-LUAD, TCGA-LUSC, and TCGA-SKCM, patients were classified into two groups based on the median FPKM value of GBP1.Differentially expressed genes (DEGs) were calculated between the groups for each tumor type using DESeq2 [30] with raw count data.Significant genes were defined when |log2FoldChange|≥ 2 and adjusted p value ≤ 0.05.Gene ontology enrichment analysis of the biological processes of DEGs was performed using the clusterProfiler package [31].

Clinical features of tissue microarrays
Two cohorts of patients with NSCLC were included in this study.Cohort 1 contained 253 paraffin-embedded tumor tissues retrospectively collected at the Department of Oncology and Department of Pathology, Tongji Hospital (Wuhan, China).Cohort 2 retrospectively acquired baseline tumor tissues from 128 patients treated with immunotherapies-based treatments and followed-up with CT imaging to evaluate treatment response and survival.The treatment response was evaluated based on iRECIST [32].The study was approved by the ethics board of Tongji Hospital, Huazhong University of Science and Technology (TJIRB20200731).Informed consent for this retrospective analysis was waived.

Immunohistochemistry assays
The sections were deparaffinized with xylene and rehydrated in 100, 90, 80, and 70% ethanol for 10 min.The slides were then rinsed under running distilled water for 3 min.Antigen retrieval was processed with steaming slides in sodium citrate buffer heated at 99-100 °C for 20 min.The slides were removed from the heat and allowed to stand at room temperature in the buffer for 20 min.Endogenous peroxidase activity was analyzed using 3% hydrogen peroxide.After washing with 1 × TBST, a universal protein block was applied with bovine serum albumin.Then, the slides were stained with the monoclonal mouse anti-Human GBP1 Antibody (1:200, clone 4D10, LS-B4320; LSBio, Inc., Seattle, WA, USA) and the recombinant rabbit monoclonal to CD8 alpha antibody (1:1000, clone EPR22483-288, ab245118; Abcam, Cambridge, MA, USA) in phosphate-buffered saline and incubated at 4 °C overnight.The next day, the sections were cooled naturally to room temperature and incubated with the HRP-conjugated secondary antibody against the primary antibody.Then, the slides were immunostained with a peroxidase/diaminobenzidine (DAB) rabbit/mouse Bio Detection Kit (Kit-9710, DAB-0031; Maixin-Bio, Fuzhou, China).Subsequently, the sections were counterstained with hematoxylin (Zymed Laboratories, San Francisco, CA, USA) for 5 min and covered with a coverslip.The slides were scanned using CaseViewer software.DAB stain intensities were analyzed using the CaseViewer TMA function.The relationship between GBP1 and CD8 + T-cell infiltration was assessed using the Pearson method.Overall survival was analyzed using Kaplan-Meier curves with the log-rank test.

Statistical analysis
Differences between two groups were analyzed using Student's t-test.Univariate Cox analysis was used to define the hazard ratio of GBP1 in the ICI-treated cohorts.Statistical significance was set at a p value < 0.05.Analyses were conducted using R (version 4.0.5) and GraphPad (version 8.0) software.
Next, we investigated the impact that the IFN-α/β/IFN-γ/ IL-15 pathways have on tumor shrinkage.Here, we introduced the objective response to evaluate the magnitude of tumor shrinkage.Patients in each cohort were classified into responders and non-responders based on outcome measurements (''responder'' is defined as a complete response [CR] or PR, and ''non-responder'' defined as SD or PD [33]).Almost all high-expression pathway groups had a higher ORR than that in the low-expression group (Fig. 1b).Similar to the ORR, the percentage of responders was higher in the high-expression group (Fig. 1c).These results indicate that higher expression of these pathways may support a better clinical response.Sustained effects of immunotherapy are crucial for long-term survival.We then investigated whether the IFN-α/β/IFN-γ/IL-15 pathways could predict longterm survival.Patients with higher expression of the three pathways displayed a higher OS of > 36 months (Fig. 1d).Cumulatively, these data suggest that the IFN-α/β/IFN-γ/ IL-15 pathways could predict the response and survival in patients treated with ICB.

GBP1 positively correlates with the IFN-α/β/IFN-γ/ IL-15 pathways
As each pathway contains multiple genes, it is inconvenient and costly to fully examine them in clinical practice.Thus, we aimed to identify a single gene that could represent these three pathways.We analyzed transcriptomes from the TCGA database.The correlation coefficients between each gene and the three pathways were calculated for 33 cancer types.In the list, GBP1 was found to rank first (Fig. 2a).GBP1 is a GTPase belonging to the dynamin superfamily, which could be provoked by IFN-γ signaling [34].As CD8 + T-cell is the primary producer of IFN-γ, the expression of GBP1 in the context of immunotherapy may reflect the active adaptive immunity.This finding supports the positive correlation results obtained in this study.Then, we confirmed the association between GBP1 and the IFN-α/β/IFN-γ/IL-15 pathways in the immunotherapy cohorts.GBP1 was positively correlated with IFN-α/β/IFN-γ/IL-15 pathways (allρ > 0.5, p < 0.001) (Fig. 2b).To extend this finding, we confirmed this in the GEO datasets, which also showed that GBP1 was positively correlated with the IFN-α/β/IFN-γ/IL-15 pathways (Supplement Figure S2).This suggests that the positive correlation observed is likely a universal phenomenon.IFN-α/β/IFN-γ/IL-15 pathways are involved in the development of CD8 + T cells [11], and we have found that GBP1 positively correlated with them.Thus, we aimed to characterize the immune context of the TME with GBP1 expression.It has been reported that the ratio of pro-inflammatory cytokines to immunosuppressive cytokines and the ratio of CD8 + T cells to Tregs, tumor-associated macrophages (TAMs), and MDSCs are related to the responsiveness to ICB immunotherapy [35].
Pro-inflammatory and immunosuppressive cytokines were significantly elevated in tumors with high GBP1 expression, which suggests that GBP1 could support anti-tumor immunity.Further investigation revealed that the ratio of CD8 + T cells to Tregs, TAMs, and MDSCs was significantly higher in tumors with high GBP1 expression (Fig. 2c).This suggests that GBP1 could promote the infiltration of CD8 + T cells, thereby creating an immunosupporting microenvironment.GSEA supported these findings, showing that GBP1 enriched IL-2 signaling and the inflammatory response, indicating an activated immune response (Supplement Figure S3).We further applied multiple gene signatures to evaluate the responsiveness to immunotherapy, which revealed that patients with high GBP1 expression tended to be more sensitive to immunotherapy (Fig. 2d).Collectively, the results suggest that GBP1 expression could be used to represent the IFN-α/β/IFN-γ/IL-15 pathways, and that GBP1 indicates an immuno-hot TME.

GBP1 expression in baseline tumor tissues correlates with improved overall survival and treatment response
IFN-α/β/IFN-γ/IL-15 pathways were associated with improved survival and response in patients treated with ICIs, and GBP1 could serve as a surrogate to IFN-α/β/ IFN-γ/IL-15 activity in pan-cancer scale analysis.We investigated whether GBP1 alone produced similar results.We analyzed survival differences between GBP1 hi and GBP1 lo patients in the immunotherapy cohorts (Fig. 3a).Patients expressing higher GBP1 mRNA levels exhibited significantly prolonged survival rates in the Gide cohort (HR = 0.30 [0.13-0.69],p = 0.0028).In the Liu, Riaz, and van Allen cohorts, higher GBP1 levels tended to be associated with longer survival, but did not reach statistical significance.In the pooled cohort, GBP1 showed significantly prolonged survival rates.The clinical response was further compared between the high and low GBP1 groups.In all cohorts, the ORR was much higher in the high GBP1 group (Fig. 3b), which also had more responders than those in the low GBP1 group (Fig. 3c).Together, these results show that GBP1 could predict survival and clinical response in patients treated with ICB, and the predictive effect was comparable with that of the IFN-α/β/ IFN-γ/IL-15 pathways.
Multiple studies have reported that immune scores and immune-related signatures are predictive biomarkers for clinical responses [36][37][38].To determine the universal predictive and prognostic potential of GBP1 in comparison with other methods, we analyzed the hazard ratio of GBP1 and existing scoring systems and signatures in the immunotherapy cohorts [23,24,26].The hazard ratio of GBP1 was comparable with those of these signatures, indicating that GBP1 had a comparable predictive effect (Fig. 3d).We further analyzed the area under the receiver operating characteristic curve (AUC) values for GBP1 expression and existing biomarkers, including the most commonly used biomarker, CD274 and TMB.The average AUC of GBP1 was higher than that of CD274 and TMB (Fig. 3e).The comparative analysis of GBP1 with the existing biomarkers suggested that GBP1 could serve as a potential indicator of immunotherapy.Furthermore, GBP1 is a single biomarker that is easily detected, thus making it more applicable in clinical practice than existing immunerelated signatures.

GBP1 is mainly expressed in macrophages and associated with functional T cells
GBP1 is involved in the host immune response to exotic cells and can be induced by IFN expression [34].The role of GBP1 in tumors is complex, and both anti-and pro-tumor effects have been reported.To determine the specific cell type expressing GBP1 that contributes to immunotherapyresponsive tumors, we collected published single-cell RNA sequencing data from patients with melanoma and NSCLC.In a melanoma dataset [18], GBP1 was rarely expressed in tumor cells, whereas it was comparably enriched in macrophages, as well as CD8 + T, CD4 + T, NK, and endothelial cells (Fig. 4a and b).In the NSCLC datasets [17], we found a similar expression pattern, with macrophages and endothelial cells displaying higher GBP1 expression levels (Fig. 4c and d).These results suggest that GBP1 is seldom expressed in tumor cells, but highly expressed in immune cells.To identify cell types expressing GBP1, we conducted a pan-cancer analysis, including melanoma and NSCLC, to evaluate GBP1 expression in 25 immune cell types.Similarly, GBP1 was mainly expressed in monocytes and macrophages (Fig. 4e and Supplement Figure S4).Macrophages have been reported to account for a large proportion of the immune cells present in the TME.These results suggest that macrophages are the primary cell type that expresses GBP1.To elucidate the function of tumors highly expressing GBP1, we conducted functional analysis.GSEA showed that T-cell migration and activation were significantly upregulated across multiple tumors (Fig. 4f-h).T-cell infiltration of the TME depends on key chemokines.And macrophage-derived CXCL9 and CXCL10 are important for ICB-induced antitumor immunity [39].The results of GSEA, together with the previous findings, suggested that GBP1 expression in tumors may promote CD8 + T-cell migration.

GBP1 is positively correlated with key chemokines that direct CD8 + T-cell migration
T-cell migration to the TME is dependent on multiple chemokines, including CCL5, CXCL9, and CXCL10 [40,41].To confirm the relationship between GBP1 and T-cell migration, we compared T-cell migration scores based on GBP1 expression.Patients with high GBP1 expression displayed significantly higher T-cell migration scores (Fig. 5a).We then focused on the above three chemokines and found that GBP1 was positively correlated with CCL5, CXCL9, and CXCL10 across 33 cancer types in the TCGA (Fig. 5b).We analyzed immunotherapy cohorts and observed the same phenomenon (Fig. 5c).This suggests that the positive correlation between GBP1 and the key chemokines was consistent across multiple cohorts, indicating that GBP1 may recruit CD8 + T cells by enhancing the production of CCL5, CXCL9, and CXCL10.

GBP1 expression is related to CD8 + T-cell infiltration and response to immune checkpoint blockade in clinical cohorts
The abovementioned results suggest a positive role for GBP1 in anti-tumor immunity and immunotherapy in multiple tumor types.To validate these findings in clinical samples, we collected two cohorts of lung cancer tissues to construct tissue microarrays.GBP1 expression and CD8 + T-cell infiltration were detected via immunohistochemistry (IHC) analysis.In cohort 1, patients were divided into high and low GBP1 expression groups.CD8 + T-cell infiltration was significantly higher in the high GBP1 expression group (Fig. 6a and b).Correlation analysis revealed that GBP1 expression was positively correlated with CD8 + T-cell infiltration (Fig. 6c).This is in accordance with the previous bioinformatics findings.This result supports previous findings that GBP1 is likely an indicator of CD8 + T-cell infiltration.
To validate the association between GBP1 expression and treatment response and survival after immunotherapy, we further analyzed the relationship between GBP1 expression and clinical response in cohort 2. The results suggested that GBP1 expression staining in biopsies from patients with PR or SD was higher than in those from patients with PD (Fig. 6d).The patients were stratified into high and low GBP1 expression groups based on the median expression of GBP1.Overall survival of the high GBP1 expression group was also significantly longer than that of the low-expression group (HR = 0.327 [0.1759-0.6078],p = 0.0012) (Fig. 6e).These results are in accordance with those of the previous analyses of public data and indicate that GBP1 could serve as a potential prognostic marker for immunotherapy.

Discussion
It is becoming increasingly clear that immune responses to ICB are influenced by multiple factors, including tumor mutations and immune response biomarkers.PD-L1, TMB alone, or these in combination have been tested as predictive biomarkers in clinical trials and showed promising results [42][43][44].Although these biomarkers have been correlated with responses to ICB, problems still exist.Standardization of PD-L1 staining and the optimal cutoff of TMB led to incomplete enrichment of responses.In addition, the multitude of networks and cellular phenotypes in the TME have not been considered.Ayers et al. developed T-cell-inflamed gene expression profiles (GEPs) related to antigen presentation, chemokine expression, cytotoxic activity, and adaptive immune resistance [23].This immune signature was validated in KEYNOTE-028 and could predict the response to pembrolizumab in multiple tumor types [45].However, optimization in terms of GEP calculations in individual patients and the economic cost also hinder its use in a clinical setting.Therefore, there is an increased demand for using currently available methods to develop a single gene with immediate translational potential.
In this study, we initially investigated three immunerelated pathways (IFN-α/β/IFN-γ/IL-15 pathways) that are involved in the functional development of CD8 + T cells [11].Type I IFN is released by almost all cells in the body, and cancer cells serve as one of its sources in tumors [46,47].It plays a critical role in anti-tumor immunity by supporting CD8 + T-cell activation [9,48].The previous studies identified type I IFN as a predictive biomarker in patients with breast carcinoma that was treated with anthracycline-based chemotherapy [49].Recombinant type I IFN has also been approved as an anticancer treatment [50,51].However, the role of type I IFN in predicting immunotherapy response and survival has not yet been reported.IFN-γ is a primary effector cytokine of cytotoxic CD8 + T cells, which has been reported to highly correlate with clinical response to therapy in melanoma [52] and breast cancer [53].IL-15 is a cytokine primarily released by dendritic cells, monocytes, and epithelial cells.It functions as a critical factor for homeostatic proliferation and survival of memory CD8 + T cells [12].Combination therapy of IL-15 with anticancer monoclonal antibodies has entered clinical trials [54].The current findings suggest that IFN-α/β/IFN-γ/IL-15 pathways may serve as predictive markers in immunotherapy.Thus, we explored their predictive effect in the immunotherapy cohorts.As expected, patients with higher expression levels exhibited better clinical responses and survival.These results demonstrated that the IFN-α/β/IFN-γ/IL-15 pathways likely confer sensitivity to ICB and could serve as predictive markers.However, it is inconvenient and costly to detect all the molecules involved in these pathways in the clinic.Therefore, we investigated whether a single gene could represent these pathways.Based on the TCGA project data, we identified GBP1 as a gene positively correlated with the IFN-α/β/ IFN-γ/IL-15 pathways, which indicated that GBP1 could specifically represent these pathways.As IFN-α/β/IFN-γ/ IL-15 pathways are involved in the activation and memory of CD8 + T cells [11], we analyzed the relationship of GBP1 with CD8 + T cells in the TME.GBP1 was significantly associated with CD8 + T-cell infiltration and the hot TME.To confirm the above results, we stained CD8 + T cells in tissue microarrays of lung cancer.A positive correlation between GBP1 expression and CD8 + T-cell infiltration was observed.These results indicate that GBP1 could reflect the infiltration of CD8 + T cells in patients.
Survival analysis revealed that higher expression of GBP1 improved survival rates.The number of responders was much higher in the high GBP1 expression group.These results were in accordance with analysis of the IFN-α/β/ IFN-γ/IL-15 pathways.We found a comparable predictive effect of GBP1 with that of previously reported signatures and biomarkers.In addition, GBP1 is a single biomarker and could more easily be detected with IHC than with TMB, T-cell clonality, etc.To test the predictive effect of GBP1, we conducted GBP1 staining in tissue microarrays of patients with lung cancer who had been treated with immunotherapy.
Based on the expression level of GBP1, we found that the overall survival was also longer in the high GBP1 expression group.These results support the potential of using GBP1 as a biomarker.
GBP1 is a critical IFN-stimulated gene that can be activated by type I or II IFNs and plays an important role in innate immunity [34].As GBP1 can be induced by IFN signaling, patients with high GBP1 expression may reflect active IFN signaling, which may represent the active anti-tumor immunity.The previous studies found that PD-L1 expression is frequently driven by IFN-γ exposure, which type I IFNs can also induce [55].Intratumoral PD-L1 expression can be considered an indirect measure of local T-cell activity [56].However, this reflects only the transient status.GBP1 has been reported to moderate the reaction to IFN signaling and inhibit cellular sensitivity to apoptosis in response to inflammatory stimuli [57].This may support long-term T-cell function, which is in accordance with the function of the IL-15 pathway.The expression of GBP1 may not only reflect the activation of CD8 + T cells, but may also reveal the sustained effect of CD8 + T cells.Thus, GBP1 could more comprehensively reflect the functional role of CD8 + T cells than PD-L1 can.However, the role of GBP1 in anti-tumor immunity has not yet been reported.In addition, some studies have shown that tumor cells expressing GBP1 can promote tumor growth [58].Thus, the observed predictive effect of GBP1 raised the question of which cells expressed GBP1, and how they contribute to anti-tumor immunity.Singlecell datasets were used to analyze the cell distribution of GBP1.It was found that GBP1 was mainly expressed in macrophages but not tumor cells.Macrophages are a heterogeneous subtype that includes anti-tumor M1 and protumor M2 macrophages [59].The predominant expression of GBP1 in macrophages indicates that it supports their antitumor role.GSEA revealed that T-cell migration and activation signaling were enriched.The previous studies have shown that macrophages produce CXCL9 and CXCL10, which are key chemokines that direct CD8 + T cells [60].
A recent study also demonstrated that macrophage-derived CXCL9 and CXCL10 are pivotal for ICB [39].Our study found a strong correlation between GBP1 and chemokines that direct T cells.These results suggest that GBP1 in tumors may promote chemokine production, thus enhancing CD8 + T-cell infiltration.In summary, this study aimed to identify a gene that reflects the entire course of CD8 + T-cell development during anti-tumor immunity.IFN-α/β/IFN-γ/IL-15 pathways were chosen for initial analysis, and we found that GBP1 could represent them.The predictive effects of GBP1 were tested and validated in discovery and validation cohorts.This study comprehensively analyzed the role and potential function of GBP1 in anti-tumor immunity and immunotherapy.Further studies are needed to investigate how GBP1 influences CD8 + T-cell recruitment and its potential role as a therapeutic target.

Fig. 1
Fig. 1 Expression of IFN-α/β/IFN-γ/IL-15 pathways is associated with prolonged survival rates and improved clinical response.a Kaplan-Meier estimates of overall survival (OS) were stratified according to expression of the IFN-α/β/IFN-γ/IL-15 pathways in the four immunotherapy and pooled cohorts.b Objective response rates

Fig. 3
Fig. 3 GBP1 in the baseline tumor tissue correlates with favorable responses to immune checkpoint blockade.a Overall survival (OS) of patients treated with immune checkpoint inhibitors according to GBP1 expression.b Objective response rates (ORRs) were observed in the immunotherapy and pooled cohorts for each group.c Percent-

Fig. 4
Fig. 4 GBP1 is mainly expressed in macrophages and correlated with T-cell migration and activation.a Uniform Manifold Approximation and Projection (UMAP) and b violin plots displaying cell-type annotations and GBP1 expression in single cells of the melanoma patient cohort.c UMAP and d violin plots exhibiting cell-type annotations

Fig. 5
Fig. 5 Correlation between GBP1 expression and key chemokines that direct CD8 + T-cell migration.a T-cell migration score between patients with high and low GBP1 expression.b and c Correlation of

Fig. 6
Fig. 6 GBP1 expression was related to CD8 + T-cell infiltration and the response to immune checkpoint blockade in clinical cohorts.a Representative images of GBP1 expression and CD8 + T-cell infiltration in cohort 1. Scale bar: 50 μm.b Density of CD8 + T cells in the high and low GBP1 expression groups.c Correlation between GBP1